Objective To understand the cument situation in cleaning and packaging of ophthalmic microsurgical instruments in hospitals in China,and provide basis for putting forward specific solutions and implementing the guidelines for cleaning ophthalmic instruments. Methods Questionnaire survey was used to analyze the problems in cleaning of ophthalmic microsurgical instruments and sterile packaging materials in 170 hospitals in China. Results In 170 hospitals,55（32.4%） had equipped with special cleaning machines for ophthalmic microsurgical instruments. Ophthalmic microsurgical instruments were included in the management of central sterile supply departments in 104（61.2%） hospitals,61（35.9%） manually cleaned ophthalmic microsurgical instruments,111（65.3%） used enzymatic detergents to clean ophthalmic microsurgical instruments. Of the 113 hospitals using silicone oil,34（30.1%） used alkaline detergents and 12（10.6%） used non-medical detergents such as turpentine and cleanser essence. Non-woven（76.5%）,paper-plastic（52.9%） and cotton（42.9%） were three most used sterile packaging materials. Conclusion The allocation rate of special cleaning machine for ophthalmic microsurgical instruments in domestic hospitals is low. Methods of cleaning and selection of cleaning agents needs to be standardized. Selection of packing materials for some hospitals are simple.